If he testifies in a Senate trial of the president, it could be a blockbuster moment.
Bolton was at the center of significant White House foreign policy debates, including Trump's efforts to press Ukrainian President Volodymyr Zelenskiy to open investigations of one of Trump's top 2020 Democratic challengers, former Vice President Joe Biden, his son Hunter's work for a Ukrainian natural gas company and a debunked conspiracy theory that Ukraine attempted to undermine Trump's 2016 election campaign.
He also said proposed having acting White House Chief of Staff Mick-Mulvaney, his adviser, Robert Blair and Office of Management and Budget official Michael Duffey testify.
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As a top White House aide who witnessed numerous events that prompted the House of Representatives to impeach Trump in December, Bolton could provide new evidence about Trump's efforts to pressure Ukraine to investigate a political rival.
The House sought his testimony but ultimately never subpoenaed Bolton, and Democrats withdrew their subpoena for his former deputy after it was challenged in court, as Democrats wanted to move forward with their impeachment probe and not wait for the court's decision. The Republicans hold the Senate with a majority of 53 seats, and the Democrats would have to find four Republicans to vote with to issue a subpoena. However, there is a possibility that several Democratic senators will join Republicans.
Why it matters: Bolton's testimony could offer key insights to unanswered questions at the heart of the impeachment inquiry - namely, why President Trump withheld military aid to Ukraine while seeking to pressure the country to investigate his political rivals.
Bolton released his statement after a court decided not to rule on whether he was obliged to testify.
The White House could try to block Bolton from testifying.
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Whether the Senate ends up hearing from Mr Bolton or not, Mr Trump is unlikely to be removed from office. "Bolton refused to testify in the House, following Trump's orders".
Remember, the GOP leader has at least paid lip-service to the idea of the chamber holding a fair and credible impeachment trial, but McConnell has also made clear he does not want to see any additional witnesses called who could speak directly to Trump's innocence or guilt.
"As House Democrats continue their political delay, they're searching desperately for some new talking points to help them deflect blame for what they've done", McConnell said.
"Speaker Pelosi can not dictate how the Senate operates", Senator Ted Cruz said in the release.
"Bolton is an important witness to misconduct involving Ukraine that he called a 'drug deal", Schiff said.
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According to The Hill, McConnell has the votes to keep the Senate trial short, meaning new witness testimony and documents would not be included.